Root-O-Mat

Features

We developed a software tool enabling user-friendly and standardized pre- and post-processing of images of rooted soil by combining image processing techniques such as image registration, calibration, and segmentation in a graphical user interface. The added benefits of this image processing approach include an improved workflow in soil zymography. For evaluation, we conducted a rhizobox experiment with maize and determined the activity of leucine-aminopeptidase before and after glucose addition based on soil zymography. The temporal and spatial distribution of enzyme activity at the root-soil interface can be visualized by Root-o-Mat which offers 1) standardized image pre-processing, 2) calibration, 3) identification of hotspots of various intensity thresholds, 4) spatial analysis for selected roots, 5) inter-active illustration of enzyme activity profile lines, 6) image viewer, and 7) detection of temporal changes of enzyme activity. Registering images of the same rhizobox taken in successive periods allows further temporal and spatial analysis. We conclude that Root-o-Mat simplifies and firmly anchors image processing and image analyses in soil zymography.

HINT: You will be able to scroll the images by clicking on the arrows below or by dragging the images. You can also click on an image which will open a gallery with more images.

Image Manager

Load, duplicate, and label your images.

Preprocessing

Rotate or flip your images if incorrectly aligned. Use Root-o-mat features such as:

  • Conversion to grey scale
  • Image registration
  • Image histogram viewer

Calibration

Choose your area of interest and number of calibration points (manual data entry), select a type of calibration curve:

  • Linear
  • Exponential and polynomic fit
  • Basic fit statistic

Analysis

Let Root-o-mat analyze for you: The tool provides 2D image processing via graphical user interface, it calculates and visualizes:

  • Change detection
  • Clustering
  • Hot spot and cold spot detection

Download

HINT: An email containing the download link will be send to you automatically after you will enter your email address and clicking on the download button.

Root-O-Mat Download:

Root-O-Mat Manual:

User manual for Root-O-Mat. Click Here

Root-O-Mat Presentation:

Here you will find a short presentation of Root-O-Mat. Click Here

Root-O-Mat Source Code:

The source code will be available via git repository soon.

Contact

HINT: By clicking on the CONTACT button, you can send us a message via contact form.

If you find any bug or just have an awesome idea that we could consider to implement in our next release, do not hesitate to contact us.

Contact